Journal article
S. Shaposhnikov, Chatarina Larsson, S. Henriksson, A. Collins, M. Nilsson
Mutagenesis, 2006
Mutagenesis, 2006
Semantic Scholar
DOI
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APA
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Shaposhnikov, S., Larsson, C., Henriksson, S., Collins, A., & Nilsson, M. (2006). Detection of Alu sequences and mtDNA in comets using padlock probes. Mutagenesis.
Chicago/Turabian
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Shaposhnikov, S., Chatarina Larsson, S. Henriksson, A. Collins, and M. Nilsson. “Detection of Alu Sequences and MtDNA in Comets Using Padlock Probes.” Mutagenesis (2006).
MLA
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Shaposhnikov, S., et al. “Detection of Alu Sequences and MtDNA in Comets Using Padlock Probes.” Mutagenesis, 2006.
BibTeX Click to copy
@article{s2006a,
title = {Detection of Alu sequences and mtDNA in comets using padlock probes.},
year = {2006},
journal = {Mutagenesis},
author = {Shaposhnikov, S. and Larsson, Chatarina and Henriksson, S. and Collins, A. and Nilsson, M.}
}
Abstract
Single cell gel electrophoresis, or the comet assay, is widely used to measure DNA damage and repair. However, the behaviour of the DNA under the conditions used for the comet assay is not fully understood. In developing a method for studying specific gene sequences within comets, using 'padlock probes' (circularizable oligonucleotide probes), we have first applied probes that hybridize to Alu repetitive elements and to mitochondrial DNA (mtDNA). During the sequence of stages in the comet assay, mtDNA progressively disperses into the surrounding agarose gel, showing no tendency to remain with nuclear DNA in the comets. In contrast, Alu probes remain associated with both tail and head DNA.
